Checklists

1. Outer box intact: no crush damage, punctures, water marks or opened tape.
2. Coolant state noted: gel packs still cold, or dry ice still present (record which).
3. Temperature indicator or data logger read and saved, if one was included.
4. Contents match the packing list: compound, quantity, vial count and lot number.
5. Each vial sealed: cap and crimp intact, no cracks, no powder outside the vial.
6. Powder appearance noted: dry cake or loose powder, no visible liquid or collapsed, glassy residue.
7. COA received for every lot, and the lot number on the COA matches the vial label.
8. Receipt logged: date, time, receiver, lot, condition and storage location.
9. Material moved to its storage band within the time your procedure allows.

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1. Unit type confirmed: manual-defrost or ultra-low freezer for frozen stock, not a frost-free unit.
2. Continuous temperature monitoring in place, with a calibrated probe and an alarm.
3. Probe placed where the samples sit, not by the door or the evaporator.
4. Box map created: positions, compound, lot and aliquot IDs.
5. Desiccant included for lyophilized material, with a humidity indicator if possible.
6. Light-sensitive material in amber vials or an opaque secondary box.
7. Back-up location agreed in case the unit fails.
8. Inventory record updated with the location.

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1. Number and size of aliquots planned from the expected use per experiment.
2. Vial equilibrated to room temperature in a desiccator before opening.
3. Clean workspace prepared; gloves on; tools and tubes sterile or clean as required.
4. Low-binding tubes chosen if the peptide is hydrophobic, cationic or dilute.
5. Labels printed before dispensing: compound, lot, aliquot ID, concentration, date, initials.
6. Aliquots dispensed with minimal headspace; inert-gas overlay used for oxidation-prone peptides.
7. Aliquots frozen promptly in their storage band.
8. Box map and inventory updated, including how many aliquots exist.

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1. Peptide content checked on the COA (net peptide content or salt form) so the concentration is calculated correctly.
2. Solvent chosen from the peptide's charge and hydrophobicity; a small test portion dissolved first if solubility is uncertain.
3. Vial equilibrated to room temperature before opening.
4. Solvent added down the vial wall; mixed by gentle swirling or inversion rather than vortexing hard.
5. Solution inspected: clear, no particles or haze.
6. pH checked where it matters for stability or the assay.
7. Sterile-filtered through a low-binding 0.2 µm filter if the application needs it.
8. Labeled and logged with concentration, solvent, date, initials and use-by date.

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1. COA identifies the compound, the lot or batch number and the date of analysis.
2. Lot on the COA matches the lot on the vial.
3. Identity test reported (usually mass spectrometry), with observed mass close to the expected mass.
4. Purity method named (usually RP-HPLC), with the detection wavelength and the result as area percent.
5. Chromatogram and mass spectrum attached or available on request.
6. Content or salt information given (net peptide content, counterion, water) where relevant.
7. Testing laboratory named, and whether it is in-house or third-party.
8. Signature or approval from a responsible person, and a date.
9. COA filed with the receipt record for the lot.

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1. Temperature records reviewed for excursions since the last audit.
2. Alarm tested and contact list confirmed.
3. Frost build-up checked; defrost scheduled if needed.
4. Inventory spot-checked against the box map.
5. Expired or past use-by aliquots removed and disposal recorded.
6. Labels checked for legibility and adhesion.
7. Desiccant checked and replaced if saturated.
8. Audit signed and dated.

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