Handling guides
Every guide, by section
Laboratory handling guides for research peptides: storage, light and moisture, freeze/thaw, containers, labeling, cold chain, solution preparation, COAs, HPLC and mass spectrometry.
Storage fundamentals
How peptides degrade, and how temperature, light, moisture and freeze/thaw cycles decide how long material stays usable.
- ST-01 How Peptides Degrade: The Chemistry Behind Every Storage Rule Oxidation, deamidation, hydrolysis and aggregation explain why peptides are kept cold, dry, dark and sealed. Knowing which pathway threatens a sequence tells you which precaution matters… −20 °C2–8 °C15–25 °C
- ST-02 Lyophilized Peptides: What the Powder Is and Why It Lasts Freeze-drying removes the water that drives most degradation. Here is what a lyophilized cake is, what can go wrong with it, and how to keep it… −20 °C2–8 °C
- ST-03 Temperature Considerations: Choosing the Right Band Why −20 °C is the default for peptide powder, when −80 °C is worth it, what 2–8 °C is good for, and how to think about… −80 °C−20 °C2–8 °C15–25 °C
- ST-04 Light Exposure: Which Peptides Need Protecting and How Ultraviolet and short-wavelength visible light can oxidize aromatic and sulfur-containing residues. How to tell whether a peptide is at risk, and what amber glass and opaque… −20 °C2–8 °C
- ST-05 Moisture and Humidity: Keeping Dry Material Dry Water undoes the benefit of lyophilization. Where moisture gets in, why opening a cold vial is the biggest risk, and how to use desiccants properly. −20 °C2–8 °C15–25 °C
- ST-06 Freeze/Thaw Cycles: Why Every Thaw Counts Freezing a peptide solution is not a neutral pause. Ice formation concentrates solutes, shifts pH and creates interfaces. How damage accumulates, and how to design a… −80 °C−20 °C
- ST-07 Short-Term Storage: Days to Weeks How to hold peptide material that will be used soon: sealed powder in active use, working solutions for the current week, and material sitting on the… −20 °C2–8 °C15–25 °C
- ST-08 Long-Term Storage: Months to Years Keeping peptide stock usable for months or years: why powder is the best long-term form, when to use −80 °C, inert-gas overlays, secondary containment and planned… −80 °C−20 °C
Containers, labels & contamination
Choosing vials and tubes, labeling that survives the freezer, aliquoting once, and keeping samples clean.
- CT-01 Container Selection: Glass, Plastic and Peptide Adsorption Peptides stick to surfaces, and at low concentrations a large share can be lost to the tube wall. How to choose between glass, polypropylene and low-binding… −80 °C−20 °C2–8 °C
- CT-02 Laboratory Labeling: What Every Peptide Container Should Say A label is the shortest form of lab documentation. The fields every peptide vial and aliquot needs, how to make labels survive the freezer, and a… −80 °C−20 °C2–8 °C
- CT-03 Aliquoting: Split Once, Thaw Once Dividing stock into single-use portions is the most effective way to protect peptide solutions. How to size aliquots, which tubes to use, and how to keep… −80 °C−20 °C
- CT-04 Sample Contamination: Microbial, Chemical and Cross-Contamination How peptide samples get contaminated by microbes, chemicals, other peptides and the containers themselves, and the habits that prevent each one. 2–8 °C15–25 °C
Cold chain & receiving
What happens in transit, what to check when a box arrives, and how to monitor the equipment material lives in.
- CC-01 Cold Chain Considerations: Shipping and Transfer How peptides are kept in range between supplier and freezer: ambient, gel-pack and dry-ice shipping, what data loggers tell you, and how to judge a temperature… −80 °C−20 °C2–8 °C15–25 °C
- CC-02 Receiving and Inspecting Peptide Shipments A short, consistent inspection when a shipment arrives catches problems while they can still be fixed. What to check, what to record, and when to quarantine. −20 °C2–8 °C15–25 °C
- CC-03 Storage Equipment and Temperature Monitoring Choosing and running freezers and refrigerators for peptide storage: manual vs frost-free defrost, probe placement, alarms, mapping and what to do when a unit fails. −80 °C−20 °C2–8 °C
Research solution preparation
Dissolving lyophilized peptide for laboratory work: solvent choice, clean technique and how long solutions last.
- SP-01 Research Solution Preparation: Dissolving Peptides Accurately How to turn a vial of lyophilized peptide into a solution of known concentration for in vitro laboratory work: correcting for peptide content, adding solvent, mixing… 2–8 °C15–25 °C
- SP-02 Solvent Selection: Matching the Solvent to the Sequence Water works for many peptides, but not all. How to predict solubility from net charge and hydrophobicity, and when to reach for dilute acid, dilute base… 15–25 °C
- SP-03 Solution Stability: How Long a Prepared Peptide Lasts Peptides in solution degrade much faster than dry powder. The factors that set how long a solution remains usable, and how to set a defensible use-by… −80 °C−20 °C2–8 °C
Documentation & quality
Lab records, certificates of analysis, HPLC, mass spectrometry, purity terms and lot traceability.
- QA-01 Laboratory Documentation for Peptide Handling What to record from receipt to disposal so that any result can be traced back to the material behind it. Borrowing the useful parts of GLP…
- QA-02 Certificates of Analysis: What a COA Is and Is Not A COA reports test results for one specific lot. What a credible certificate contains, which tests matter for peptides, and the limits of what any COA…
- QA-03 Interpreting a COA: Reading the Numbers Line by Line A practical walk-through of a peptide certificate of analysis: matching the lot, reading the HPLC trace, checking the observed mass, and understanding what purity does and…
- QA-04 Analytical Testing Overview: Which Test Answers Which Question Identity, purity, content, impurities and contaminants each need a different method. A map of the analytical techniques used for peptides and what each one can and…
- QA-05 HPLC Explained: How Peptide Purity Is Measured How reversed-phase HPLC separates a peptide from its impurities, how to read a chromatogram, and why the reported purity depends on the method.
- QA-06 Mass Spectrometry Explained: Confirming Peptide Identity How mass spectrometry weighs a peptide, how to read charge states and adducts on a spectrum, and the difference between average and monoisotopic mass.
- QA-07 Purity Terminology: Purity, Content, Counterions and Impurities Purity, net peptide content, peptide impurities, counterions and water are separate measurements that are often confused. A plain-language guide to what each term means.
- QA-08 Lot Traceability: Connecting Results to Material Every vial, aliquot and result should trace back to a lot and its certificate. How to set up a simple traceability chain in a research lab…
No guide matches that filter.