PHG · REV 2026.10 For laboratory research use only. Not medical or dosing guidance.
Peptide Handling Guide Storage, handling and documentation for research peptides
Document
CS-EPITAL
Type
Compound handling sheet
Revised
Sources checked
Bands
−20 °C

Epitalon Handling Sheet

Also known as Epithalon, Epithalone, AEDG peptide

A tetrapeptide, Ala-Glu-Asp-Gly, whose Asp-Gly pair is one of the fastest-isomerizing motifs in peptide chemistry. Dry storage is straightforward; solutions need care.

2 min read

Identity

Sequence
AEDG
Formula
C14H22N4O9
Molecular weight
390.35 g/mol (average)
Usual supplied form
Lyophilized powder
Record
PubChem

M oxidation-prone N deamidation-prone D Asp-Gly / Asp-Ser site

Formula and weight are for the free peptide. Salt forms (acetate, TFA) weigh more; use the figure on the lot's COA.

Storage & sensitivity

Lyophilized−20 °C
In solution−20 °C
Oxidation
Low
Light
Low
Moisture
Moderate
Inferred from structure

Little compound-specific stability data is published. Guidance follows from the sequence and general peptide chemistry.

Bench card

  • The Asp-Gly pair isomerizes readily in solution, forming isoaspartate.
  • Keep solutions cold, slightly acidic and short-lived; freeze aliquots for anything longer.
  • Acidic (net charge about −2 at neutral pH); dissolves easily in water.
  • No oxidation-prone residues; light risk is low.
  • Isomers have the same mass, so mass spectrometry alone will not detect them.

Handling-relevant features

FeatureWhy it matters for handling
Asp3-Gly4Classic fast isomerization site through a succinimide intermediate; produces isoaspartate
Glu2, Asp3, C-terminusAcidic overall; very water-soluble
No aromatic or sulfur residuesLow oxidation and light risk

What it is

Epitalon is a synthetic tetrapeptide, Ala-Glu-Asp-Gly, studied mainly by Khavinson and colleagues. Its literature can be found through PubMed.

The Asp-Gly site

An aspartate followed by glycine is one of the most reactive sites in peptide chemistry. Geiger and Clarke showed that aspartyl residues form a succinimide intermediate that reopens to give a mixture of normal aspartate and isoaspartate, and that the small glycine side chain makes ring formation especially easy. In Epitalon this site is a quarter of the molecule.

Storage

Form Recommended Notes
Sealed powder −20 °C Desiccant; the dry solid is relatively robust
Solution, days 2–8 °C Slightly acidic; use promptly
Solution, longer −20 °C or colder Single-use aliquots

References

  1. PubMed literature on Epitalon (Ala-Glu-Asp-Gly), a synthetic tetrapeptide. PubMed
  2. Geiger T, Clarke S. Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides: succinimide-linked reactions that contribute to protein degradation. J Biol Chem. 1987;262(2):785–794. PubMed
  3. Robinson NE, Robinson AB. Molecular clocks. Proc Natl Acad Sci USA. 2001;98(3):944–949. DOI
  4. Patel K, Borchardt RT. Chemical pathways of peptide degradation. II. Kinetics of deamidation of an asparaginyl residue in a model hexapeptide. Pharm Res. 1990;7(7):703–711. PubMed

This sheet covers laboratory storage and handling of research material only. It does not describe use in humans or animals, and the supplier's datasheet for your lot takes priority.